Transcriptomic Profiling of Glioblastoma Samples

The mRNA expression profiles of tumor samples from Patient 1 and the 50 additional GBM patients were generated using Agilent 4 × 44 K Whole Human Genome Oligo Microarrays (Agilent, USA). The data were normalized using the quantile method of the “limma”^{31 (link)} R package. All of the raw and processed data are available from the GEO database (accession numbers GSE83300 and GSE83301). The raw mRNA expression data and the corresponding clinical information, which was available for 487 GBM samples, were downloaded from the Cancer Genome Atlas (TCGA) database (https://tcga-data.nci.nih.gov/tcga/tcgaHome2.jsp) and processed using the RMA methodology included in the “affy”^{32 (link)} R package.