For validation of mature miRNAs expression, 200 ng of total RNA was reverse transcribed in vitro to cDNA using the MystiCq microRNA cDNA Synthesis Mix (Sigma-Aldrich, Saint Louis, MO, USA) according to the manufacturer’s instructions. Relative expression was calculated using the Ct method with uninfected as the reference and small nuclear RNA U6 as endogenous controls.
JEV RNA was quantified as described earlier14 (link). Similarly, RNA was extracted from JEV infected mice brain and uninfected control brain. Notch1, Dll1, JAG1, Hes1 expression were examined with specific primer by qRT-PCR method. The RNA transcript levels were normalized to that of
JEV RNA was quantified as described earlier14 (link). Similarly, RNA was extracted from JEV infected mice brain and uninfected control brain. Notch1, Dll1, JAG1, Hes1 expression were examined with specific primer by qRT-PCR method. The RNA transcript levels were normalized to that of
Free full text: Click here
