Real-time cell migration assay

The procedure was carried out as previously described [20 (link), 26 (link)]. Briefly, 4 × 10⁴ of each of serum-starved DU145 stable cells were suspended in 100 μl of serum-free medium, and then cell suspension was added into the pre-equilibrated upper chamber of CIM-plate. The lower chamber was filled with 160 μl of complete DMEM containing 10% FBS. The kinetic cell index of migration was recorded every 15 min for 24 h and then calculated by RTCA software v1.2 (Roche Applied Science).

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Yuan H., Deng R., Zhao X., Chen R., Hou G., Zhang H., Wang Y., Xu M., Jiang B, & Yu J. (2017). SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis. Molecular Cancer, 16, 157.

Publication 2017

RTCA software v1.2

Kinetic Rtca Serum

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University

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Variable analysis

independent variables

Serum-starved DU145 stable cells

dependent variables

Kinetic cell index of migration

control variables

Number of serum-starved DU145 stable cells (4 × 10^4)
Volume of serum-free medium (100 μl)
Volume of complete DMEM containing 10% FBS in the lower chamber (160 μl)
Time period of measurement (24 h)
Measurement interval (every 15 min)

controls

No positive or negative controls were explicitly mentioned in the protocol.

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