Biomimetic Composite Scaffold Fabrication

Sodium alginate (SA, biochemical grade, MW = 438,000), polyvinyl alcohol (PVA, 98%, alcoholysis degree: 87.0–89.0 mol%), hydroxyapatite (HAP, 98%), D-glucono-δ-lactone (GDL, 99%), 2-methylpyridine borane complex (2-PBC, 95%), and Arg-Gly-Asp (RGD, 97%) were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Alginate dialdehyde (ADA) with theoretical oxidation degrees of 10% was synthesized by specific oxidation of sodium periodate [67 (link)]. Cellulose nanocrystals (CNCs) were acquired by sulfuric acid hydrolysis of microcrystalline cellulose based on our previous report [77 (link)]. Other biochemical reagents, including MC3T3-E1 cells, culture medium, antibiotics, and assay kits, were purchased from Gibco, Thermo Fisher Scientific Co., Ltd. (Waltham, MA, USA).

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Wang H., Yin R., Chen X., Wu T., Bu Y., Yan H, & Lin Q. (2023). Construction and Evaluation of Alginate Dialdehyde Grafted RGD Derivatives/Polyvinyl Alcohol/Cellulose Nanocrystals IPN Composite Hydrogels. Molecules, 28(18), 6692.

Publication 2023

MC3T3-E1 cells culture medium

2 methylpyridine Alginate Antibiotics Assay Borane Cells Cellulose Culture medium Hydrolysis Hydroxyapatite Lactone Microcrystalline cellulose Polyvinyl alcohol Sodium alginate Sodium periodate Sulfuric acid

Corresponding Organization : Hainan Normal University

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Variable analysis

independent variables

Sodium alginate (SA, biochemical grade, MW = 438,000)
Polyvinyl alcohol (PVA, 98%, alcoholysis degree: 87.0–89.0 mol%)
Hydroxyapatite (HAP, 98%)
D-glucono-δ-lactone (GDL, 99%)
2-methylpyridine borane complex (2-PBC, 95%)
Arg-Gly-Asp (RGD, 97%)
Alginate dialdehyde (ADA) with theoretical oxidation degrees of 10%
Cellulose nanocrystals (CNCs)

dependent variables

Not explicitly mentioned

control variables

MC3T3-E1 cells
Culture medium
Antibiotics
Assay kits

positive controls

Not specified

negative controls

Not specified

Annotations

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