Quantifying Anti-HPV16 L1 IgG Titers

The anti-HPV16 L1 IgG titers of mouse sera were measured as described [13] (link), [14] . Briefly, 96-well ELISA plates were coated overnight with 100 ng of purified HPV16 L1 VLPs per well and blocked with 5% skim milk in PBST. The plates were reacted with three- or four-fold serial dilutions of mouse sera for 1 h at 37°C. The anti-HPV16 L1 IgG bound to the coated HPV16 L1 VLPs was detected using HRP-conjugated goat anti-mouse IgG antibody (Bethyl), and color reactions were developed as described above.

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Kim H.J., Jin Y, & Kim H.J. (2014). The Concentration of Carbon Source in the Medium Affects the Quality of Virus-Like Particles of Human Papillomavirus Type 16 Produced in Saccharomyces cerevisiae. PLoS ONE, 9(4), e94467.

Publication 2014

HRP-conjugated goat anti-mouse IgG antibody

Anti igg Antibody Dilutions Elisa Goat Hpv16 Milk Mouse Sera

Corresponding Organization : Chung-Ang University

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Variable analysis

independent variables

Three- or four-fold serial dilutions of mouse sera

dependent variables

Anti-HPV16 L1 IgG titers

control variables

96-well ELISA plates coated overnight with 100 ng of purified HPV16 L1 VLPs per well
Plates blocked with 5% skim milk in PBST
Plates reacted with three- or four-fold serial dilutions of mouse sera for 1 h at 37°C
Anti-HPV16 L1 IgG bound to the coated HPV16 L1 VLPs detected using HRP-conjugated goat anti-mouse IgG antibody (Bethyl)
Color reactions developed as described above

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