Synovial Tissue Immunohistochemistry for RA

Synovium was collected by closed Parker-Pearson needle biopsy from one actively inflamed knee joint in RA patient (Schumacher & Kulka, 1972 (link)). As described previously (Ma et al., 2014 (link); Zou et al., 2013 (link)), serial sealed sections of qualified synovial samples were stained with hematoxylin and eosin (H&E) and also immunohistochemically stained with the commercial mouse monoclonal antibodies (Invitrogen, Carlsbad, CA, USA) according to standard staining protocols: anti-CD3 (clone PS1, T cells), anti-CD15 (clone My1, neutrophils), anti-CD20 (clone L26, B cells), anti-CD34 (clone QB End/10, vascular endothelial cells), anti-CD38 (clone SPC32, plasma cells), and anti-CD68 (clone KP1, macrophages). Irrelevant isotype was used as a negative control. Appropriate positive controls were included to rule out the possibility of an absence of staining due to technical failure.

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Chen Y.L., Lin J.Z., Mo Y.Q., Liang J.J., Li Q.H., Zhou C.J., Wei X.N., Ma J.D., Yang Z.H., Zheng D.H, & Dai L. (2018). Joint damage is amplified in rheumatoid arthritis patients with positive thyroid autoantibodies. PeerJ, 6, e4216.

Publication 2018

mouse monoclonal antibodies anti-CD3 anti-CD20 anti-CD34 anti-CD38 anti-CD68

Anti cd3 B cells Clone Eosin Isotype Knee joint Macrophages Monoclonal antibodies Mouse Needle biopsy Neutrophils Patient Plasma cells Serial Synovium T cells Vascular endothelial cells

Corresponding Organization :

Other organizations : Sun Yat-sen Memorial Hospital, Sun Yat-sen University

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Variable analysis

independent variables

Synovium collection method (closed Parker-Pearson needle biopsy)

dependent variables

Presence and distribution of cell types (T cells, neutrophils, B cells, vascular endothelial cells, plasma cells, macrophages) in synovial samples

control variables

RA patient with actively inflamed knee joint

controls

Positive controls: Included to rule out the possibility of an absence of staining due to technical failure.
Negative control: Irrelevant isotype used.

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