Yeast Spore Viability Assay

Spore viability was performed as previously described by Le et al. (2013 (link)) with minor modifications. Strains were mated on SPA for 48–72 hr. Asci were treated with 1:500 glusulase (Perkin Elmer, Waltham, MA) and incubated at room temperature overnight. Spore count was determined by a hemocytometer and 2000, 5000, or 10,000 spores of wild type, rec12Δ, or mutant, respectively, were plated over 10 YES plates. Spore viability was determined as the number of colonies formed over the number of plated spores. Spore viability was normalized to levels observed for the wild type.

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Blyth J., Makrantoni V., Barton R.E., Spanos C., Rappsilber J, & Marston A.L. (2017). Genes Important for Schizosaccharomyces pombe Meiosis Identified Through a Functional Genomics Screen. Genetics, 208(2), 589-603.

Publication 2017

glusulase

Glusulase Spore Spore count Strains

Corresponding Organization : Wellcome Centre for Cell Biology

Other organizations : Technische Universität Berlin

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Variable analysis

independent variables

Strains (wild type, rec12Δ, mutant)

dependent variables

Spore viability

control variables

Spore count (2000, 5000, or 10,000 spores plated)
Incubation time (48–72 hr for mating, overnight for glusulase treatment)
Plating medium (YES plates)

controls

Positive control: wild type spores
Negative control: Not explicitly mentioned

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