CFU assays were performed with MethoCult GF (M3434, Stem Cell Technologies) according to the manufacturer’s instructions as described previously [23 (link), 24 (link)].
NOS2−/− and WT mice received 6 Gy IR. Bone marrow cells were harvested and placed into 3434 methylcellulose medium 14 days after IR. The CFU for granulocytes and macrophages (CFU-GM) and burst-forming-units for erythroid cells (BFU-E) were measured 7 days later, and the CFUs for granulocytes, erythrocytes, monocytes, and megakaryocytes (GEMM) were measured 12 days later. The colony-forming units were calculated using 105 BMCs. Sections with more than 30 cells were scored as a colony under an inverted microscope according to the manufacturer’s instructions.
NOS2−/− and WT mice received 6 Gy IR. Bone marrow cells were harvested and placed into 3434 methylcellulose medium 14 days after IR. The CFU for granulocytes and macrophages (CFU-GM) and burst-forming-units for erythroid cells (BFU-E) were measured 7 days later, and the CFUs for granulocytes, erythrocytes, monocytes, and megakaryocytes (GEMM) were measured 12 days later. The colony-forming units were calculated using 105 BMCs. Sections with more than 30 cells were scored as a colony under an inverted microscope according to the manufacturer’s instructions.
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