In vivo imaging, spleen volume, cardiac function and relaxometry were performed as described in L. Jackson et al.23 (link), at 30 weeks of age using a 9.4T MR system (Agilent Technologies, Santa Clara, USA) equipped with 1000 mT/m gradient inserts and a 39mm volume resonator RF coil (RAPID Biomedical, Rimpar, Germany). A small animal physiological monitoring system (SA Instruments, Stony Brook, NY) was used to record the ECG trace, respiration rate, and internal temperature. Animals were anaesthetized with a mixture of isoflurane and oxygen with physiological measurements used to maintain the depth of anaesthesia23 (link).
Shangaris P., Loukogeorgakis S.P., Subramaniam S., Flouri C., Jackson L.H., Wang W., Blundell M.P., Liu S., Eaton S., Bakhamis N., Ramachandra D.L., Maghsoudlou P., Urbani L., Waddington S.N., Eddaoudi A., Archer J., Antoniou M.N., Stuckey D.J., Schmidt M., Thrasher A.J., Ryan T.M., De Coppi P, & David A.L. (2019). In Utero Gene Therapy (IUGT) Using GLOBE Lentiviral Vector Phenotypically Corrects the Heterozygous Humanised Mouse Model and Its Progress Can Be Monitored Using MRI Techniques. Scientific Reports, 9, 11592.
Other organizations :
University College London, National Center for Tumor Diseases, South African Medical Research Council, University of the Witwatersrand, University of Cambridge
Physiological monitoring system (ECG, respiration, temperature)
Anesthesia (isoflurane and oxygen)
controls
Positive control: none mentioned
Negative control: none mentioned
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