Cellular Viability Assay Protocol

Cellular viability assays were performed as previously described [21 (link),22 (link)]. Briefly, cells were seeded into opaque black 96-well plates. To determine the cellular viability of siRNA-treated cells, reverse lipid-based transfections were performed (RNAiMAX, life technologies, Carlsbad, CA, USA). Following defined incubation periods, cellular viability was assessed using the CellTiter-Blue^® reagent (Promega) in a plate reader (VICTOR^TM X4, Perkin Elmer, Waltham, MA, USA). All experiments were conducted as technical and biological triplicates.

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Flebbe H., Spitzner M., Marquet P.E., Gaedcke J., Ghadimi B.M., Rieken S., Schneider G., Koenig A.O, & Grade M. (2022). Targeting STAT3 Signaling Facilitates Responsiveness of Pancreatic Cancer Cells to Chemoradiotherapy. Cancers, 14(5), 1301.

Publication 2022

RNAiMAX CellTiter-Blue VICTORTM X4

Assays Biological Cells Cellular viability Lipid Promega Sirna Transfections

Corresponding Organization :

Other organizations : University of Göttingen

Top 5 similar protocols

Variable analysis

independent variables

SiRNA treatment

dependent variables

Cellular viability

control variables

Seeding cells into opaque black 96-well plates
Reverse lipid-based transfections (RNAiMAX, life technologies, Carlsbad, CA, USA)
Incubation periods
CellTiter-Blue® reagent (Promega)
Plate reader (VICTOR™ X4, Perkin Elmer, Waltham, MA, USA)

controls

All experiments were conducted as technical and biological triplicates

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