Quantitative Protein Analysis by Western Blot

Western blot analyses were performed as previously described (22 (link)). Immunoblotting was carried out with antibodies against human BRCA-1 (catalog no. 9010), DNMT-1 (catalog no. 5119), cyclin D1 (catalog no. 92G2), phospho-p53-(Ser20) (catalog no. 9287), and GAPDH (catalog no. 2118) obtained from Cell Signaling Technology, and ERα (catalog no. sc-542) obtained from Santa Cruz Biotechnology. Immunocomplexes were detected by using enhanced chemiluminescence (GE Healthcare Life Sciences). The GAPDH protein was used as an internal control for normalization of protein expression.

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Romagnolo D.F., Donovan M.G., Papoutsis A.J., Doetschman T.C, & Selmin O.I. (2017). Genistein Prevents BRCA1 CpG Methylation and Proliferation in Human Breast Cancer Cells with Activated Aromatic Hydrocarbon Receptor. Current Developments in Nutrition, 1(6), e000562.

Publication 2017

BRCA-1 DNMT-1 cyclin D1 phospho-p53-(Ser20) GAPDH enhanced chemiluminescence

Antibodies Brca 1 Chemiluminescence Cyclin d1 Dnmt Gapdh Human Protein Western blot analyses

Corresponding Organization : University of Arizona

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Variable analysis

independent variables

BRCA-1
DNMT-1
Cyclin D1
Phospho-p53-(Ser20)

dependent variables

Protein expression

control variables

GAPDH protein

controls

No positive or negative controls were explicitly mentioned.

Annotations

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