Live-cell imaging of PLK4-driven mitosis

Leica DMI8 SP8 microscope and LASX software was used for confocal imaging, live-cell imaging and image processing. For live-cell imaging, PLK4 overexpressing U2OS-H2B-mCherry cells were synchronized by double-thymidine treatment [53 (link)], metaphase cells were tracked and recorded based on the fate of division. Metaphase scoring was performed using Carl Zeiss Axio Imager M1. Competition assays were performed by using BioTek Cytation 5 Cell Imaging Multimode Reader.

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Kalkan B.M., Ozcan S.C., Cicek E., Gonen M, & Acilan C. (2024). Nek2A prevents centrosome clustering and induces cell death in cancer cells via KIF2C interaction. Cell Death & Disease, 15(3), 222.

Publication 2024

DMI8 SP8 microscope Axio Imager M1 Cytation 5 Cell Imaging Multimode Reader

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Other organizations : Koç University

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