Transcriptome Analysis of Locust Flight Muscle

The flight muscle of three independent replicates was collected for tissue preparation. Total RNA extraction was performed using a TRIzol reagent (Invitrogen), and cDNA libraries were prepared in accordance with the protocols of Illumina. Raw data were filtered, corrected, and mapped to locust genome sequence via HISAT software. The gene expression levels were measured using the criteria of reads per kb per million mapped reads. Differentially expressed genes (DEGs) were detected using the EdgeR software. The differences between the treatment and control groups were represented by p-values and FDR. The DEGs with significance levels at FDR <0.05 in each comparison were considered as the candidate target genes (Jiang et al., 2019a (link)). The fastq files of the transcriptome sequence are available at BioProject PRJNA690129. The full-length transcriptome was obtained from Jiang et al., 2019b (link), with NCBI accession number PRJNA517220. Briefly, the full-length transcripts from testes of locusts were enriched by 5’-Cap capturing assay for library preparation; 200 ng of the RNA libraries were loaded on FLO-MIN106 (R9.4) flowcells and were run on a MinION or a GridION X5 (Oxford Nanopore Technologies).