Platelet Function Analysis Protocol

Alteplase was purchased from Boehringer Ingelheim (Ingelheim am Rhein, Germany). Adenosine diphosphate (ADP), collagen, ristocetin, arachidonic acid and epinephrine were purchased from Helena Laboratories (Beaumont, TX, USA). Collagen-related peptide (CRP) was prepared as previously described.^{17 (link)} Fluorescein isothiocyanate (FITC)-conjugated mouse anti-human CD41a and FITC-conjugated mouse anti-human PAC-1 antibodies were purchased from BD Biosciences (San Jose, CA, USA) and Becton Dickinson (San Jose, CA, USA), respectively. Phycoerythrin (PE)-conjugated mouse anti-human CD62p (P-selectin) and purified mouse anti-human glycoprotein (GP) VI antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated mouse anti-human CD42b antibody was purchased from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). Thrombin was purchase from Sigma-Aldrich (St Louis, MO, USA).