Genetic Labeling of VIP Neurons

All experiments were approved by the University of Michigan Institutional Animal Care and Use Committee and were in accordance with NIH guidelines for the care and use of laboratory animals. Animals were kept on a 12-h day/night cycle with ad libitum access to food and water. VIP-IRES-Cre mice (Vip^{TM 1(cre)Zjh}/J, Jackson Laboratory, stock # 010908) (Taniguchi et al., 2011 (link)) were crossed with Ai14 reporter mice (B6.Cg-Gt(ROSA)26Sor^{TM 14(CAG–tdTomato)Hze}/J, Jackson Laboratory, stock #007914) (Madisen et al., 2010 (link)) to yield F1 offspring that expressed the fluorescent protein tdTomato in VIP neurons. Because mice on the C57BL/6J background undergo age-related hearing loss, experiments were restricted to mice aged P30 – P85, an age range where hearing loss should be minimal or not present (Zheng et al., 1999 (link)).

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Rivera-Perez L.M., Kwapiszewski J.T, & Roberts M.T. (2021). α3β4∗ Nicotinic Acetylcholine Receptors Strongly Modulate the Excitability of VIP Neurons in the Mouse Inferior Colliculus. Frontiers in Neural Circuits, 15, 709387.

Publication 2021

VipTM 1(cre)Zjh/J B6.Cg-Gt(ROSA)26SorTM 14(CAG–tdTomato)Hze/J

Age related hearing loss Animals C57bl mice Food Hearing loss Institutional animal care and use committee Ires Laboratory animals Mice Neurons Protein Rosa Tdtomato

Corresponding Organization : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Age of mice (P30 - P85)

dependent variables

Not explicitly mentioned

control variables

Mice were kept on a 12-h day/night cycle
Mice had ad libitum access to food and water
Mice were on the C57BL/6J background to minimize age-related hearing loss

controls

Positive control: Not specified
Negative control: Not specified

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