Spermidine was purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol was obtained from Zhiyuan Chemical Reagent Co. Ltd. (Tianjin, China). Peptide was isolated and identified as previously described [66 (link)]. Briefly, peptide was isolated by liquid chromatography tandem mass spectrometry using a reverse-phase nanocolumn (RP-nano-LC-MS/MS). Peptide sequences were identified and searched with ProteinPilot Software (AB SCIEX, Framingham, MA, USA) against a translated protein database of A. japonicus nucleotide sequences from the National Center for Biotechnology Information (NCBI). P03 was synthesized with solid-phase synthesis with a purity of >95% from Shanghai Top-peptide Biotechnology Co., Ltd. (Shanghai, China). The certificate of analysis and MS spectrum for P03 were showed in Figures S1 and S2. The mitochondrial membrane potential assay kit with JC-1(#C2006), the cell cycle and apoptosis analysis kit (#C1052), the annexin V-FITC apoptosis detection kit (#C1062L), and the reactive oxygen species assay kit (#C0033) were purchased from Beyotime Biotechnology (Shanghai, China).
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