Fibroblast Proliferation Assay with TxVIIB

The human skin normal fibroblast cell line, 1BR.3.GN, obtained from the European Collection of Authenticated Cell Cultures was confirmed to be mycoplasma free and cultured to test effects of TxVIIB on cell proliferation on a xCELLigence SP system (ACEA Bioscience) as described previously (18 (link)). A plant extract, which previously shown enhanced cell proliferation (unpublished results), was used as a positive control in the assay, serum-free media was used as a negative control. Day 4 cell proliferation rates were compared between treatment and control wells, and a one-way ANOVA test was used for multiple comparisons with Holm-Sidak’s correction, using GraphPad Prism 9.3.1.

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Raffaelli T., Wilson D.T., Dutertre S., Giribaldi J., Vetter I., Robinson S.D., Thapa A., Widi A., Loukas A, & Daly N.L. (2024). Structural analysis of a U-superfamily conotoxin containing a mini-granulin fold: Insights into key features that distinguish between the ICK and granulin folds. The Journal of Biological Chemistry, 300(4), 107203.

Publication 2024

xCELLigence SP system GraphPad Prism 9.3.1

Corresponding Organization : James Cook University

Other organizations : Institut des Biomolécules Max Mousseron, Université de Montpellier, Centre National de la Recherche Scientifique, École Nationale Supérieure de Chimie de Montpellier, University of Queensland

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Variable analysis

independent variables

TxVIIB

dependent variables

Cell proliferation rates on day 4

control variables

1BR.3.GN human skin normal fibroblast cell line
Mycoplasma-free cell culture
Serum-free media

positive control

Plant extract (previously shown to enhance cell proliferation)

negative control

Serum-free media

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