Western Blot and Immunohistochemistry Protocol

WB and IHC were performed according to established methods in the previous study [38 (link)]. Primary antibodies were used at appropriate dilution concentration as follows: WB, anti-ATG13 (Protein Tech, Chicago, IL, USA, 1:2000), anti-ATG14 (Protein Tech, 1:1000), anti-SQSTM1/p62 (Protein Tech, 1:4000), anti-BECN1 (Abcam, Cambridge, UK, 1:1000), anti-LC3B (Protein Tech, 1:2000) and β-actin (Abcam, 1:2500). IHC, anti-BECN1 (Abcam, 1:100).

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Lv Z., Qiu X., Jin P., Li Z., Zhang Y., Lv L, & Song F. (2022). MiR-371a-5p Positively Associates with Hepatocellular Carcinoma Malignancy but Sensitizes Cancer Cells to Oxaliplatin by Suppressing BECN1-Dependent Autophagy. Life, 12(10), 1651.

Publication 2022

anti-ATG14 anti-SQSTM1/p62 anti-BECN1 anti-LC3B β-actin

Actin Antibodies Becn1 Dilution Protein

Corresponding Organization : Chongqing Medical University

Other organizations : Nanchong Central Hospital, Chongqing Emergency Medical Center

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Variable analysis

independent variables

Primary antibodies used for Western blot (WB) and immunohistochemistry (IHC)

dependent variables

Protein expression levels of ATG13, ATG14, SQSTM1/p62, BECN1, and LC3B as measured by WB
BECN1 protein expression as measured by IHC

control variables

Established methods for WB and IHC from previous study [38]
Dilution concentrations of primary antibodies used for WB and IHC

controls

No positive or negative controls were explicitly mentioned in the provided information.

Annotations

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