Immunostaining of Dissociated Neural Cells

Immunostaining of dissociated neural cells was performed as described previously [74 (link),75 (link)]. The following primary antibodies were used: Chicken anti-GFP (A10262; Thermo Fisher Scientific Inc., Waltham, MA, USA), rabbit anti-GFP (A11122; Thermo Fisher Scientific Inc., Waltham, MA, USA). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (A11039, A11008; Thermo Fisher Scientific Inc., Waltham, MA, USA) were used to detect primary antibodies.

Free full text: Click here

Lee Y.J., Kim H.R., Lee C.Y., Hyun S.A., Ko M.Y., Lee B.S., Hwang D.Y, & Ka M. (2020). 2-Phenylethylamine (PEA) Ameliorates Corticosterone-Induced Depression-Like Phenotype via the BDNF/TrkB/CREB Signaling Pathway. International Journal of Molecular Sciences, 21(23), 9103.

Publication 2020

Chicken anti-GFP rabbit anti-GFP A11122 Alexa Fluor dyes A11039

Antibodies Chicken Dyes Neural cells Rabbit

Corresponding Organization : Korea Research Institute of Chemical Technology

Other organizations : Pusan National University, Korea Brain Research Institute

Top 5 similar protocols

Variable analysis

independent variables

Immunostaining of dissociated neural cells

dependent variables

Expression of GFP detected by chicken anti-GFP and rabbit anti-GFP antibodies

control variables

Experimental protocol followed as described previously [74, 75]
Appropriate secondary antibodies conjugated with Alexa Fluor dyes used to detect primary antibodies

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!