Protein Expression and Regulation Analysis

Total protein was extracted and protein concentration was detected by BCA assay. Western blot analysis was performed as previously described [39 (link)]. EFNB2 (ab69858, Abcam, Cambridge, UK), EPHB4 (ab150545, ab98933, Abcam), LDLR (ab52818, Abcam), β-catenin (ab32572, Abcam), VLDLR (ab203271, Abcam), SCARB1(ab52629, Abcam), STAT3(ab68153, Abcam), p-STAT3(ab267373, Abcam), JAK2(ab108596, Abcam), p-JAK2(ab108596, Abcam), SREBP2(ab30682, Abcam), proliferating cell nuclear antigen (PCNA; Proteintech Group, Inc., Sankt Leon-Rot, Germany) primary antibodies were used. Horseradish peroxidase (HRP)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (SA00001-2) and HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (SA00001-1) were obtained from Proteintech Group, Inc (Chicago, US).

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Xu C., Gu L., Kuerbanjiang M., Jiang C., Hu L., Liu Y., Xue H., Li J., Zhang Z, & Xu Q. (2022). Adaptive activation of EFNB2/EPHB4 axis promotes post-metastatic growth of colorectal cancer liver metastases by LDLR-mediated cholesterol uptake. Oncogene, 42(2), 99-112.

Publication 2022

ab69858 ab150545 ab98933 ab52818 ab32572 ab52629 ab68153 ab267373 ab108596 ab30682 PCNA Horseradish peroxidase HRP)-conjugated Affinipure Goat Anti-Rabbit IgG (H + L) (SA00001-2) HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L) (SA00001-1

Anti igg Antibodies Assay Goat Horseradish peroxidase Jak2 Ldlr Mouse Pcna Protein Rabbit Stat3 Vldlr Western blot analysis β catenin

Corresponding Organization : Ministry of Health of the Russian Federation

Other organizations : Shanghai Cancer Institute, State Key Laboratory of Oncogene and Related Genes

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Variable analysis

independent variables

Total protein extraction
Protein concentration detection by BCA assay

dependent variables

Expression of EFNB2
Expression of EPHB4
Expression of LDLR
Expression of β-catenin
Expression of VLDLR
Expression of SCARB1
Expression of STAT3
Phosphorylation of STAT3
Expression of JAK2
Phosphorylation of JAK2
Expression of SREBP2
Expression of PCNA

control variables

Western blot analysis as previously described [39]

Annotations

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