Assessing Absorption of Gorse Compounds

In a previous publication by Iyer et al. (2021) (link), ten compounds were found to be significantly higher in Gorse compared to other invasive plants found in Scotland, namely, p-coumaric acid, acetovanillone, protocatechuic acid, chlorogenic acid, vanillin, p-hydroxybenzoic acid, sinapic acid, kaempferol, mandelic acid and ferulic acid. Among these however, only six compounds, namely, p-coumaric acid, acetovanillone, vanillin, p-hydroxybenzoic acid, mandelic acid and ferulic acid were detected in the final GLPC and were used as representative sample compounds to study their metabolism as they passed along the digestion model. A standard compound mixture (50 mM, 200 µL, p-coumaric acid, acetovanillone, protocatechuic acid, chlorogenic acid, vanillin, p-hydroxybenzoic acid, sinapic acid, kaempferol, mandelic acid and ferulic acid) was prepared and incubated with the Caco-2 cells for 24 h at 37 °C to assess active cell transport.