Longitudinal In Vivo Microcomputed Tomography

Longitudinal in vivo microcomputed tomography (μCT) imaging was performed at indicated timepoints using a VivaCT40 (Scanco, Nokomis, FL, United States) at a source voltage of 55 kV, a source current of 145 μA, and an isotropic voxel size of 38.0 μm. Mice were imaged under isoflurane anesthesia. Only the lower limb was imaged to reduce radiation exposure from multiple scans. Three-dimensional images were reconstructed using Scanco microCT V6.1 software. For quantification, the heterotopic bone was reconstructed separately from skeletal bone, with a lower threshold of 220 Hounsfield and an upper threshold of 1,000 Hounsfield units (Culbert et al., 2014 (link)).

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Brewer N., Fong J.T., Zhang D., Ramaswamy G, & Shore E.M. (2021). Gnas Inactivation Alters Subcutaneous Tissues in Progression to Heterotopic Ossification. Frontiers in Genetics, 12, 633206.

Publication 2021

VivaCT40 microCT V6.1 software

A 145 Anesthesia Heterotopic Isoflurane Lower limb Mice Microct Radiation exposure Scans Skeletal bone

Corresponding Organization : University of Pennsylvania

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Variable analysis

independent variables

Timepoints

dependent variables

Heterotopic bone volume

control variables

Isotropic voxel size (38.0 μm)
Source voltage (55 kV)
Source current (145 μA)
Isoflurane anesthesia
Imaging only the lower limb

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