Helicobacter pylori Infection in Mice

Thirty-nine C57BL/6N female mice certified as Helicobacter spp-free were acquired from Harlan Laboratories^® and housed at the Laboratorio de Ensayos Biológicos (LEBi), University of Costa Rica, at standard temperature (25.5°C ± 1.20°C) and humidity (50–70%), and maintained on a 12-h light/dark cycle (lights off at 6:00 p.m). Water and food (LabDiet 5010.) were provided ad libitum. Mice were distributed into 7 experimental groups, each consisting of 5-7 animals per cage (Figure 1) and were moved to an experimental section one week before they were inoculated with H. pylori. Experimental procedures and methods were carried out in accordance with the guidelines of the Costa Rican Ministry of Science and Technology and were approved by the Institutional Committee for Animal Care and Use of Animals (CICUA) of the University of Costa Rica (permission number CICUA-031-17).