Volatile
compounds present in 4 g of process waters were first collected by
dynamic headspace purge-and-trap technique, after which they were
dispersed in 10 mL of deionized water and purged (37 °C) with
nitrogen (260 mL/min flow rate) for 30 min and trapped on Tenax tubes.
Water vapor was removed with nitrogen (5 mL N/min) for 20 min prior
to volatile desorption. Trapped volatiles were desorbed and separated
by GC (Agilent Technologies 6890 N, Santa Clara, CA, USA) with a DB
1701 capillary column (30 m; i.d. 0.25 mm; 1 μm film thickness)
and an oven program as follows: initial temperature 45 °C for
5 min, after which it was increased gradually at 1.5 °C/min to
55 °C, 2 °C/min to 90 °C, and 8 °C/min to 230
°C and held for 8 min at 230 °C. The individual volatiles
were analyzed by MS (Agilent 5973 Network Mass Selective Detector)
70 eV ionization mode and a m/z scan
range between 30 and 250. Compound identification was aided by the
MS-library. Quantification was made by calibration curves of external
standards. The LOD was found to be at 5 ng/mL.
compounds present in 4 g of process waters were first collected by
dynamic headspace purge-and-trap technique, after which they were
dispersed in 10 mL of deionized water and purged (37 °C) with
nitrogen (260 mL/min flow rate) for 30 min and trapped on Tenax tubes.
Water vapor was removed with nitrogen (5 mL N/min) for 20 min prior
to volatile desorption. Trapped volatiles were desorbed and separated
by GC (Agilent Technologies 6890 N, Santa Clara, CA, USA) with a DB
1701 capillary column (30 m; i.d. 0.25 mm; 1 μm film thickness)
and an oven program as follows: initial temperature 45 °C for
5 min, after which it was increased gradually at 1.5 °C/min to
55 °C, 2 °C/min to 90 °C, and 8 °C/min to 230
°C and held for 8 min at 230 °C. The individual volatiles
were analyzed by MS (Agilent 5973 Network Mass Selective Detector)
70 eV ionization mode and a m/z scan
range between 30 and 250. Compound identification was aided by the
MS-library. Quantification was made by calibration curves of external
standards. The LOD was found to be at 5 ng/mL.
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