Lipoprotein Cholesterol and Liver Lipid Analysis

4 h-fasting plasma total cholesterol (TC), free cholesterol (FC) (Wako), and triglyceride (TG) (Roche) were determined by enzymatic analysis. Plasma was fractionated by FPLC to determine cholesterol distribution among the lipoprotein classes (32 (link)). Liver lipids were extracted with chloroform: methanol (2:1), and the extract was used for enzymatic assays (33 (link)) (cholesterol, Wako; TG, Roche), and lipids were normalized to wet liver weight. Macrophage cholesterol content was measured by gas-liquid chromatography (34 (link)) and normalized to cellular protein (34 (link)).

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Zhao Q., Wang Z., Meyers A.K., Madenspacher J., Zabalawi M., Zhang Q., Boudyguina E., Hsu F.C., McCall C.E., Furdui C.M., Parks J.S., Fessler M.B, & Zhu X. (2021). Hematopoietic Cell-Specific SLC37A2 Deficiency Accelerates Atherosclerosis in LDL Receptor-Deficient Mice. Frontiers in Cardiovascular Medicine, 8, 777098.

Publication 2021

cholesterol

Cellular Chloroform Cholesterol Enzymatic Enzymatic assays Gas liquid chromatography Lipids Lipoprotein cholesterol Liver Macrophage Methanol Plasma Protein Triglyceride

Corresponding Organization :

Other organizations : Wake Forest University, National Institute of Environmental Health Sciences

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Variable analysis

independent variables

4 h-fasting plasma

dependent variables

Total cholesterol (TC)
Free cholesterol (FC)
Triglyceride (TG)
Cholesterol distribution among the lipoprotein classes
Liver lipids
Macrophage cholesterol content

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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