Protein samples were extracted from mouse lungs for Western blot analysis as previously described.21 (link) The rabbit monoclonal antibodies of p-Stat3 (Tyr705; Cell Signaling Technology; 1:1000 dilution), p-Stat5 (Tyr694; Cell Signaling Technology; 1:1000 dilution), SOCS1 (Cell Signaling Technology; 1:1000 dilution), and GAPDH (Cell Signaling Technology; 1:1000 dilution) were used as the primary antibodies. HRP-conjugated anti-rabbit IgG (AB Clonal, Wuhan, China) was used as the second antibody. The chemiluminescent detection and image analysis were carried out as previously described.21 (link)
Protocol detail
Western Blot Analysis of Protein Signaling
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Anti igg
Antibodies
Antibody
Clonal
Dilution
Gapdh
Lungs
Monoclonal antibodies
Mouse
Protein
Rabbit
Stat3
Stat5
Western blot analysis
Corresponding Organization :
Other organizations : Jiangsu University, Affiliated Hospital of Jiangsu University, Taixing People's Hospital, Jintan People's Hospital
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Variable analysis
independent variables
- Protein samples extracted from mouse lungs
- Expression levels of p-Stat3 (Tyr705)
- Expression levels of p-Stat5 (Tyr694)
- Expression levels of SOCS1
- Expression levels of GAPDH
- Rabbit monoclonal antibodies of p-Stat3 (Tyr705; Cell Signaling Technology; 1:1000 dilution)
- Rabbit monoclonal antibodies of p-Stat5 (Tyr694; Cell Signaling Technology; 1:1000 dilution)
- Rabbit monoclonal antibodies of SOCS1 (Cell Signaling Technology; 1:1000 dilution)
- Rabbit monoclonal antibodies of GAPDH (Cell Signaling Technology; 1:1000 dilution)
- HRP-conjugated anti-rabbit IgG (AB Clonal, Wuhan, China)
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