Antibody Detection and Visualization Protocol

The antibodies used in the present study were rabbit polyclonal antibody against rainbow trout GR (1:250) [29 (link)], Caveolin-1 mouse monoclonal antibody (1:500) [30 (link),31 (link)], ORAI-1 rabbit polyclonal antibody (1:250) [22 (link),32 (link),33 (link)], and phalloidin conjugated TRITC (1:500) for F-actin detection (1:500) [34 (link)]. The secondary antibodies used were Alexa 488 goat anti-rabbit IgG and Alexa 594 donkey anti-mouse IgG (Invitrogen, Burlington, ON, Canada).

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Das C, & Vijayan M.M. (2023). Cortisol Rapidly Facilitates Glucocorticoid Receptor Translocation to the Plasma Membrane in Primary Trout Hepatocytes. Biology, 12(2), 311.

Publication 2023

Alexa 488 goat anti-rabbit IgG Alexa 594 donkey anti-mouse IgG

Alexa 594 Anti igg Antibodies Antibody Caveolin 1 Donkey F actin Goat Monoclonal antibody Mouse Phalloidin Rabbit Rainbow trout Tritc

Corresponding Organization : University of Calgary

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Variable analysis

independent variables

Rabbit polyclonal antibody against rainbow trout GR (1:250)
Caveolin-1 mouse monoclonal antibody (1:500)
ORAI-1 rabbit polyclonal antibody (1:250)
Phalloidin conjugated TRITC (1:500) for F-actin detection

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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