Aortic nitric oxide bioavailability was measured as a NOS inhibitor [NG-nitro-L-arginine methyl ester (L-NAME, 1 × 10−4 M)] induced contraction of isolated rat aorta after submaximal precontraction of the vessel with phenylephrine (1 × 10−6 M) as previously described.[14 (link)] To prevent synthesis of prostaglandins, we performed the experiment in the presence of 10 mM indomethacin. The seven number of aortic ring preparation each isolated from seven animals per group were used for the experiment (i.e. N = 7).
Drugs and chemicals
L-NAME, tempol, acetylcholine, sodium nitroprusside, L-phenyephrine, and apocynin/acetovanillone were purchased from Sigma Chemical Company Inc., St Louis, MO, USA. Indomethacin was obtained as a gift sample from Sun Pharma, Chennai, India. All other chemicals used were analytical grade purchased from Himedia Laboratories Ltd, Mumbai, India.
Data analysis
Data in the manuscript are expressed as Mean ± SEM. Comparisons between groups were made using one-way ANOVA. When significance was indicated, a Student-Newman-Keuls post hoc analysis was used. Statistical significance was assumed at P < 0.05.
Drugs and chemicals
L-NAME, tempol, acetylcholine, sodium nitroprusside, L-phenyephrine, and apocynin/acetovanillone were purchased from Sigma Chemical Company Inc., St Louis, MO, USA. Indomethacin was obtained as a gift sample from Sun Pharma, Chennai, India. All other chemicals used were analytical grade purchased from Himedia Laboratories Ltd, Mumbai, India.
Data analysis
Data in the manuscript are expressed as Mean ± SEM. Comparisons between groups were made using one-way ANOVA. When significance was indicated, a Student-Newman-Keuls post hoc analysis was used. Statistical significance was assumed at P < 0.05.
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