Human colorectal adenocarcinoma Caco-2 and HT-29 cells were cultured as previously reported [28 (link),53 (link)]. HMC-1.2 cells (HMC-1.2, Merck-Millipore, Darmstadt, Germany) were plated at 2.5 × 105 cells/mL density in T25 flasks with Isove Medium, 1.2 mM α-thioglycerol (Sigma Cat No. M6145-100ML), 10% FBS (EMD Millipore Cat. No. ES-009-B) and 1× penicillin/streptomycin, and incubated at 37 °C in a humidified atmosphere with 5% CO2. The medium was replenished every 2–3 days and the cells were sub-cultured when the cell density was at 1–1.5 × 106 cells/mL.
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