For determination of telomerase activity naïve and memory CD4+ and CD8+ T-cells were isolated and cultured in advanced RPMI 1640 (GIBCO) in the presence of 30 U/mL rIL-2 (Sigma, Vienna, Austria) and CD3/CD28 Dynabeads (bead:cell ratio 1:1; Invitrogen). After 3 and 6 days, 2 × 105 cells were harvested and telomerase activity was determined using TeloTAGGG Telomerase PCR ELISAplus kit (Roche) according to ‘Telomeric Repeat Amplification Protocols (TRAP)’.21 (link)
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Telomerase Activity in T-Cell Subtypes
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Other organizations : Medical University of Graz, Innsbruck Medical University
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Variable analysis
independent variables
- Isolation and culturing of naïve and memory CD4+ and CD8+ T-cells
- Duration of cell culture (3 and 6 days)
- Telomerase activity in naïve and memory CD4+ and CD8+ T-cells
- Culture medium (advanced RPMI 1640)
- Cytokine (30 U/mL rIL-2)
- T-cell stimulation (CD3/CD28 Dynabeads at 1:1 bead:cell ratio)
- Not specified
- Not specified
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