Protocol detail

Immunoblot Analysis of Key Proteins

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Immunoblot analysis was done as previously described (19 (link)) using antibodies directed against ATDC (Santa Cruz Biotechnology), PTEN and RELA (Cell Signaling Technology, Danvers, MA), DNMT3A and DNMT3B (Santa Cruz Biotechnology), at a 1:1000 dilution. β-actin antibody (Sigma, St Louis, MD) served as a loading control.

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Palmbos P.L., Wang L., Yang H., Wang Y., Leflein J., Ahmet M.L., Wilkinson J.E., Kumar-Sinha C., Ney G.M., Tomlins S.A., Daignault S., Kunju L.P., Wu X.R., Lotan Y., Liebert M., Ljungman M.E, & Simeone D.M. (2015). ATDC/TRIM29 drives invasive bladder cancer formation through microRNA-mediated and epigenetic mechanisms. Cancer research, 75(23), 5155-5166.

Publication 2015

DNMT3A DNMT3B β-actin antibody

Actin Antibodies Antibody Dilution Dnmt3b Immunoblot analysis Pten Rela

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Variable analysis

independent variables

Antibodies directed against ATDC, PTEN, RELA, DNMT3A, and DNMT3B

dependent variables

Protein expression levels of ATDC, PTEN, RELA, DNMT3A, and DNMT3B

control variables

β-actin antibody (Sigma, St Louis, MD) as a loading control

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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