Prion Disease Diagnostic Workflow

The specimens of blood, CSF and brain tissues of the suspected patient were collected by the sentinel hospital. All samples were transported to the national reference laboratory for human prion disease in CCDC. The peripheral blood leukocytes were used for sequencing analysis of PRNP and polymorphism of codon 129, with an automatic genetic analyzer (ABI3130XL). CSF samples were obtained by routine lumbar puncture and analysed by Western immunoblot to detect the 14-3-3 protein. Brain tissues obtained from autopsy or biopsy were applied into neuropathologic assays and/or PrP^Sc detections with immunohistochemistry and/or Western blot. The standard operation procedure (SOP) of each test was well documented in the CJD surveillance program, which was described previously [4] .

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Gao C., Shi Q., Tian C., Chen C., Han J., Zhou W., Zhang B.Y., Jiang H.Y., Zhang J, & Dong X.P. (2011). The Epidemiological, Clinical, and Laboratory Features of Sporadic Creutzfeldt-Jakob Disease Patients in China: Surveillance Data from 2006 to 2010. PLoS ONE, 6(8), e24231.

Publication 2011

14 3 3 protein Assays Autopsy Biopsy Blood Brain Codon Human Immunohistochemistry Lumbar puncture Patient Peripheral blood leukocytes Polymorphism Prion disease Sequencing analysis Tissues Western blot Western immunoblot

Corresponding Organization :

Other organizations : National Institute for Viral Disease Control and Prevention

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Protocol cited in 6 other protocols

Variable analysis

independent variables

Specimen collection from the suspected patient (blood, CSF, brain tissues)

dependent variables

PRNP gene sequencing analysis and polymorphism of codon 129
Detection of 14-3-3 protein in CSF using Western immunoblot
Neuropathologic assays and/or PrPSc detections in brain tissues using immunohistochemistry and/or Western blot

control variables

Standard operation procedure (SOP) of each test as documented in the CJD surveillance program
Automatic genetic analyzer (ABI3130XL) used for PRNP gene sequencing

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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