Culturing Human Pancreatic and Endometrial Cells

Human pancreatic adenocarcinoma cells (PDAC) such as BxPC-3 and AsPC-1, and immortalized human normal endometrial fibroblast cells (T-HESC) were purchased from American Type Culture Collection (VA, USA). Human pancreatic stellate cells (PSC) were isolated from the resected pancreas tissues of patients undergoing operation for pancreatic cancer as previously described [53 (link)], under the approval by the Ethics Committee of Tohoku University School of Medicine (article#: 2009-79 and 2014-1-132), while human endometrial adenocarcinoma primary fibroblast cells (EC6/Fib) were isolated from resected endometrium tissues [54 (link)]. PDAC and EC6/Fib were maintained in RPMI-1640, while PSC were maintained in DMEM/F-12 media, all supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (Life Technologies) at 37°C in 95% humidified atmosphere of 5% CO₂.