Immunostaining of Zebrafish Samples

Animals were fixed and stained as previously described [26 ,46 (link)]. The primary antibodies used in this study include: Sox10—1:5,000 [47 ], Acetylated Tubulin 1:10,000 (Sigma), βIII Tubulin 1:1,000 (Covance), S100 1:1,000 (Dako) [48 (link)], ErbB3 1:200 (Santa Cruz) and Isl1 1:100 (Developmental Studies Hybridoma Bank). The secondary antibodies used include Alexa antibodies (Invitrogen) (1:600); goat anti-rabbit 568, goat anti-mouse 568, goat anti-rabbit 647 and goat anti-mouse 647. After staining, zebrafish animals were stored in 50% glycerol/50% 1X PBS until imaged when they were mounted under a bridged coverslip.