The phytochemical analysis of LRC was performed with an Agilent 6890 GC apparatus interfaced to an Agilent 5973 MS system equipped with an electron ionization (EI) source and autoinjector (Agilent Technologies, Inc.). GC/MS was conducted according to a previous study (21 (link)). The GC system was built with a DB-5 column (30.0 m x 0.25 mm x 0.25 µm). The temperature varied from 80°C (5 min) to 200°C at 5°C/min, and the injection volume was 2 µl. Injection was performed in the split mode adjusted to 1:5. The carrier gas was helium at 1.0 ml/min. The inlet, source and quadrupole temperatures were set at 290, 230 and 190°C, respectively. For MS detection, the EI mode with an ionization energy of 70 eV was used, with a mass range at m/z 50-800. Agilent ChemStation software (Agilent Technologies, Inc.) was used for data processing. The phytochemical compounds were identified by mass fragmentation patterns compared via Wiley Spectral Library search.
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GC-MS Analysis of Phytochemicals
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Variable analysis
independent variables
- Phytochemical analysis technique (GC/MS)
- Phytochemical compounds (identified by mass fragmentation patterns)
- Agilent 6890 GC apparatus
- Agilent 5973 MS system
- Electron ionization (EI) source
- Autoinjector
- DB-5 column (30.0 m x 0.25 mm x 0.25 µm)
- Temperature program (80°C for 5 min, then increasing to 200°C at 5°C/min)
- Injection volume (2 µl)
- Split mode adjusted to 1:5
- Carrier gas (helium at 1.0 ml/min)
- Inlet temperature (290°C)
- Source temperature (230°C)
- Quadrupole temperature (190°C)
- EI mode with ionization energy of 70 eV
- Mass range (m/z 50-800)
- Agilent ChemStation software for data processing
- Previous study (21) used as a reference for GC/MS methodology
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