Ctenopharyngodon idella kidney cells, obtained from China Center for Type Culture Collection, were cultured according to previous description (35 (link)). Fathead minnow (FHM) cell line (36 (link)) was kindly provided by Dr. Junfa Yuan, which was maintained in M199 (Gibco) supplemented with 10% FBS (Gibco), 100 U/ml of penicillin (Sigma), and 100 U/ml of streptomycin (Sigma). Both cells were incubated at 28°C with 5% CO2 humidified atmosphere.
For virus infection, CIK or FHM cells were plated for 24 h in advance and then infected with GCRV 097 stain at a multiplicity of infection of 1. After 2 h, the virus inoculum was removed, the cells were washed with PBS, and further incubated with new medium (DMEM for CIK, M199 for FHM, and no FBS). The control group was treated with PBS.
For virus infection, CIK or FHM cells were plated for 24 h in advance and then infected with GCRV 097 stain at a multiplicity of infection of 1. After 2 h, the virus inoculum was removed, the cells were washed with PBS, and further incubated with new medium (DMEM for CIK, M199 for FHM, and no FBS). The control group was treated with PBS.
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