Total DNA and RNA were extracted using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA, USA) and RNeasy Mini Kit (Qiagen), respectively, as described in Dickinson et al.17 (link) The DNA samples were treated with RNase solution (Qiagen) and Proteinase K Solution (Qiagen) at 65 °C for 10 min and RNA samples were treated with DNase I (Qiagen) for 20 min.
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