Drosophila Stocks Maintenance and Characterization

Drosophila stocks were maintained on standard fly food (25 g/L corn flour, 5 g/L lyophilized agar, 50 g/L sugar, 50 g/L fresh yeast, 2.5 mL/L Tegosept [10% in ethanol], and 2.5 mL/L propionic acid) at 25 °C in a 12 h light/dark cycle. All experiments were performed in the same standard conditions, otherwise differently specified. The following fly strains were purchased from the Bloomington Drosophila Stock Center (BDSC, Indiana University, Bloomington, IN, USA): w¹¹¹⁸ (BDSC #3605); elav-GS (BDSC #43642); UAS-TBPH (BDSC #93601); UAS-TBPH.F-L (BDSC #93781); UAS-mCD8-GFP (BDSC #30002); Su(var)3-9¹/TM3 (BDSC #6209); UAS-Su(var)3-9.lacI (BDSC #93147); UAS-hSUV39H1.HA (BDSC #84799); elav-GAL4 (BDSC #77894); egg¹⁴⁷³/SM1 (BDSC #30565). The Su(var)3-9⁶/TM6B allele was a kind gift of Gunter Reuter [49 (link)], the G9a^RG5 allele was a kind gift of Marion Delattre [53 (link)].