Western Blot Protocol for Protein Expression Analysis

Western blot analysis was done as described previously [8 (link)]. The membrane was incubated with specific antibodies: mouse monoclonal antibodies against MMP9, p-p65, p65, p-IκB, STAT3, p-STAT3, histone H1 and β-actin (1:500 dilution, Santa Cruz Biotechnology Inc. Santa Cruz, CA, USA), Goat rabbit polyclonal MMP3 (1:500 dilution, Santa Cruz Biotechnology Inc. Santa Cruz, CA, USA), rabbit polyclonal for p50, p-IKKα, IKKα, p-IKKβ, IKKβ, p-IκB and IκB (1:500 dilution, Santa Cruz Biotechnology Inc.) and iNOS and COX-2 (1:1000 dilution, Cayman Chemical, Ann Arbor, Mich, USA). The relative density of the protein bands was scanned by densitometry using MyImage (SLB, Seoul, Korea) and quantified by Labworks 4.0 software (UVP, Inc., Upland, CA, USA).

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Park M.H., Yoon D.Y., Ban J.O., Kim D.H., Lee D.H., Song S., Kim Y., Han S.B., Lee H.P, & Hong J.T. (2015). Decreased severity of collagen antibody and lipopolysaccharide-induced arthritis in human IL-32β overexpressed transgenic mice. Oncotarget, 6(36), 38566-38577.

Publication 2015

p-IκB p-STAT3 Labworks 4.0 software

Actin Antibodies Cayman Cox 2 Dilution Goat Histone h1 Ikkα Ikkβ Inos Membrane Mmp3 Mmp9 Monoclonal antibodies Mouse Protein Rabbit Stat3 Western blot analysis

Corresponding Organization :

Other organizations : Chungbuk National University, Konkuk University, Osong Medical Innovation Foundation

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Variable analysis

independent variables

Mouse monoclonal antibodies against MMP9, p-p65, p65, p-IκB, STAT3, p-STAT3, histone H1 and β-actin (1:500 dilution, Santa Cruz Biotechnology Inc. Santa Cruz, CA, USA)
Goat rabbit polyclonal MMP3 (1:500 dilution, Santa Cruz Biotechnology Inc. Santa Cruz, CA, USA)
Rabbit polyclonal for p50, p-IKKα, IKKα, p-IKKβ, IKKβ, p-IκB and IκB (1:500 dilution, Santa Cruz Biotechnology Inc.)
INOS and COX-2 (1:1000 dilution, Cayman Chemical, Ann Arbor, Mich, USA)

dependent variables

Relative density of the protein bands

control variables

Western blot analysis was done as described previously [8 (link)]

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