Protocol detail

Comprehensive Antibody Collection for DNA Damage Response

Find Similar Protocols

Antibody recognizing 53BP1 (A300-272A) and RPA32 pS (4/8) (IHC-00422) were purchased from Bethyl Laboratories (Montgomery, TX). The phospho-specific γ-H2AX antibody (JBW301) was obtained from Millipore (Billerica, MA). Mre11 (12D7), Ku70 (GTX233114) and Ku80 (GTX70485) were purchased from Genetex. Actin (C-11), BRCA1 (sc-642). RNAPII (sc-899), ATR pS 428 (sc-109912) and Rad51 (H-92) antibodies were obtained from Santa Cruz Biotech (Santa Cruz, CA). Total (2662) and pT68 Chk2 (2661) antibodies and total (2360) and pS317 (2344) Chk1 were purchased from Cell Signaling. S9.6, an antibody specific for R loops (RNA:DNA hybrids) [37 (link)], was provided by Dr. Stephen H. Leppla (NIH, Bethesda, MD). Antibodies used for ChIP: anti-BRCA1 (Gene-Tex, 6B4), anti-ATM (Novus Biologicals NB100-305), anti-γ-H2AX (Abcam, ab2893), anti-53BP1 (Novus Biologicals, NB100-305), anti-CtIP (Abcam) and anti-Gal4(DBD) (Santa Cruz Biotechnology, sc-577). Antibodies used in western blotting and SETX IP: anti-SETX (A301-105) from Bethyl Laboratories, anti-XRN2 (NBP1-68149) and anti-Rrp45 (NBP1-71702) from Novus Biologicals.

Free full text: Click here

Morales J.C., Richard P., Patidar P.L., Motea E.A., Dang T.T., Manley J.L, & Boothman D.A. (2016). XRN2 Links Transcription Termination to DNA Damage and Replication Stress. PLoS Genetics, 12(7), e1006107.

Publication 2016

Rad51 (H-92) NB100-305 anti-53BP1 anti-CtIP anti-Gal4(DBD)

53bp1 A301 Actin Antibodies Antibody Biologicals Brca1 Brca1 gene Chip Ctip Hybrids Ku70 Novus Rnapii Setx

Top 5 similar protocols

Variable analysis

independent variables

Antibody recognizing 53BP1 (A300-272A)
RPA32 pS (4/8) (IHC-00422)
Phospho-specific γ-H2AX antibody (JBW301)
Mre11 (12D7)
Ku70 (GTX233114)
Ku80 (GTX70485)
Actin (C-11)
BRCA1 (sc-642)
RNAPII (sc-899)
ATR pS 428 (sc-109912)
Rad51 (H-92)
Total (2662) and pT68 Chk2 (2661) antibodies
Total (2360) and pS317 (2344) Chk1
S9.6, an antibody specific for R loops (RNA:DNA hybrids)
Anti-BRCA1 (Gene-Tex, 6B4)
Anti-ATM (Novus Biologicals NB100-305)
Anti-γ-H2AX (Abcam, ab2893)
Anti-53BP1 (Novus Biologicals, NB100-305)
Anti-CtIP (Abcam)
Anti-Gal4(DBD) (Santa Cruz Biotechnology, sc-577)
Anti-SETX (A301-105)
Anti-XRN2 (NBP1-68149)
Anti-Rrp45 (NBP1-71702)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

S9.6, an antibody specific for R loops (RNA:DNA hybrids)

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!