In deeply anesthetised rats—intraperitoneal [ip] injection of 40 mg/kg thiopental (Rotexmedica, Trittau, Germany) and 10 mg/kg diazepam (Apaurin; Krka, Novo Mesto, Slovenia)—we simultaneously completely occluded the end of the superior mesenteric vein (ligation) just below the joining of the lienal vein as well as close to abdominal aorta the complete occlusion of the superior mesenteric artery. Thereby, permanent occlusion leads to permanent alteration of arterial and venous blood flow and a progressive disease course.
Treatments were given intraperitoneally (1 mL/rat via an abdominal bath) at 1 min after ligation: 10 µg/kg BPC 157, 10 ng/kg BPC 157 or 5 mL/kg saline. All rats were sacrificed 30 min after ligation.
For venography, the treatments (10 µg/kg BPC 157, 10 ng/kg BPC 157 or 5 mL/kg saline) were applied intraperitoneally, as 1 mL/rat via an abdominal bath, 15 min after ligation, just before venography.
Brain swelling was recorded in rats 15 min after the complete calvariectomy was performed. Briefly, six burr holes were drilled in three horizontal lines, all of them medial to the superior temporal lines and temporalis muscle attachments. The two rostral burr holes were placed just basal from the posterior interocular line, the two basal burr holes were placed just rostral to the lambdoid suture (and transverse sinuses) on both sides, respectively) and the middle two burr holes were placed in the line between the basal and rostral burr holes.
Rats were laparatomized for the corresponding presentation of the peripheral veins (superior mesenteric, inferior mesenteric, inferior anterior pancreaticoduodenal, jejunal, middle colic, left colic, portal and inferior caval) and arteries (superior mesenteric artery, proximal and distal to occlusion, inferior mesenteric artery, abdominal aorta). The recording was with a camera attached to a VMS-004 Discovery Deluxe USB microscope (Veho, Dayton, OH, USA) performed until the end of the experiment, and assessed at 5, 15, and 30 min after ligation.
Treatments were given intraperitoneally (1 mL/rat via an abdominal bath) at 1 min after ligation: 10 µg/kg BPC 157, 10 ng/kg BPC 157 or 5 mL/kg saline. All rats were sacrificed 30 min after ligation.
For venography, the treatments (10 µg/kg BPC 157, 10 ng/kg BPC 157 or 5 mL/kg saline) were applied intraperitoneally, as 1 mL/rat via an abdominal bath, 15 min after ligation, just before venography.
Brain swelling was recorded in rats 15 min after the complete calvariectomy was performed. Briefly, six burr holes were drilled in three horizontal lines, all of them medial to the superior temporal lines and temporalis muscle attachments. The two rostral burr holes were placed just basal from the posterior interocular line, the two basal burr holes were placed just rostral to the lambdoid suture (and transverse sinuses) on both sides, respectively) and the middle two burr holes were placed in the line between the basal and rostral burr holes.
Rats were laparatomized for the corresponding presentation of the peripheral veins (superior mesenteric, inferior mesenteric, inferior anterior pancreaticoduodenal, jejunal, middle colic, left colic, portal and inferior caval) and arteries (superior mesenteric artery, proximal and distal to occlusion, inferior mesenteric artery, abdominal aorta). The recording was with a camera attached to a VMS-004 Discovery Deluxe USB microscope (Veho, Dayton, OH, USA) performed until the end of the experiment, and assessed at 5, 15, and 30 min after ligation.
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