To further observe the morphological structure and growth of tumor in each group, HE staining, Caspase-3 and FANCG immunohistochemical staining were used to detect tumor in each group [17 (link), 18 (link)]. The mice were sacrificed at the end of the experiment and the tumor tissues were fixed in 10% formalin, paraffinized, and cut into 5 μm thick sections.
HE staining.
The slides were dewaxed and stained with hematoxylin–eosin for histological assessments.
Immunohistochemistry.
After microwave pre-treatment in a citrate buffer (pH 6.0; for antigen retrieval), the slides were immersed in 3% hydrogen peroxide for 20 min to block endogenous peroxidase activity. After intensive washing with PBS, the slides were incubated with Caspase-3, FANCG antibodies and then incubated with HRP-conjugated antibodies. The slides were visualized with a DAB Horseradish Peroxidase Color Development Kit (Beyotime, China), and counterstained with hematoxylin. The images were analyzed by Image-Pro Plus 6.0 software.
Cui Y., Huang W., Du F., Yin X., Feng L, & Li B. (2022). Therapeutic benefits of niraparib tosylate as radio sensitizer in esophageal squamous cell carcinoma: an in vivo and in vitro preclinical study. Clinical & Translational Oncology, 24(8), 1643-1656.
Slides subjected to microwave pre-treatment in a citrate buffer (pH 6.0) for antigen retrieval
Slides immersed in 3% hydrogen peroxide for 20 min to block endogenous peroxidase activity
Slides incubated with Caspase-3 and FANCG antibodies
Slides incubated with HRP-conjugated antibodies
Slides visualized with a DAB Horseradish Peroxidase Color Development Kit
Slides counterstained with hematoxylin
Images analyzed by Image-Pro Plus 6.0 software
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