LNA Antagomirs for miRNA-10b Targeting

The locked nucleic acid (LNA) antagomirs (anti-miR-10b), 50-ThioMC6-D/GTGTAACACGTCTATACGCCCA-30, directed against miRNA-10b and a mismatch scrambled sequence (scr-miR), 50-ThioMC6-D/CACAAATTCGGTTCTACAGGGTA-30, were synthesized by Eurogentec (Belgium). The 5′-Thiol-Modifier C6 disulfide (5′-ThioMC6) was inserted into both sequences for conjugation to magnetic nanoparticles for in vitro studies. The thiol modified oligonucleotides were activated by treatment with 3% TCEP (Tris(2-carboxyethyl)phosphine hydrochloride, Thermo Scientific Co., Rockford, IL), followed by purification with ammonium acetate/ethanol precipitation before conjugation to the nanoparticles, as described previously (Yigit et al., 2013 (link); Yoo et al., 2014a (link)).

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Chen M., Kim B., Robertson N., Mondal S.K., Medarova Z, & Moore A. (2023). Co-administration of temozolomide (TMZ) and the experimental therapeutic targeting miR-10b, profoundly affects the tumorigenic phenotype of human glioblastoma cells. Frontiers in Molecular Biosciences, 10, 1179343.

Publication 2023

Tris(2-carboxyethyl)phosphine hydrochloride

Ammonium acetate Anti mir Disulfide Ethanol Locked nucleic acid Mirna Oligonucleotides Phosphine Tcep Thiol Tris

Corresponding Organization : Michigan State University

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Variable analysis

independent variables

Locked nucleic acid (LNA) antagomirs (anti-miR-10b), 50-ThioMC6-D/GTGTAACACGTCTATACGCCCA-30, directed against miRNA-10b
Mismatch scrambled sequence (scr-miR), 50-ThioMC6-D/CACAAATTCGGTTCTACAGGGTA-30

dependent variables

Not explicitly mentioned

control variables

5′-Thiol-Modifier C6 disulfide (5′-ThioMC6) inserted into both sequences for conjugation to magnetic nanoparticles
Thiol modified oligonucleotides activated by treatment with 3% TCEP (Tris(2-carboxyethyl)phosphine hydrochloride, Thermo Scientific Co., Rockford, IL), followed by purification with ammonium acetate/ethanol precipitation before conjugation to the nanoparticles

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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