Native Equ c 1 purified from the Breed/Gender Mix horse hair extract was prepared (2‐D Clean‐Up Kit, GE Healthcare) for two‐dimensional (2D)‐gel electrophoresis that was performed with immobilized pH gradient strips using the PROTEAN i12 isoelectric focusing system (Bio‐Rad Laboratories) as previously described.
17 (link) Gels were stained with SYPRO Ruby protein gel stain (Invitrogen, Thermo Fisher Scientific) according to the manufacturer's instructions. Images were recorded using the Amersham typhoon gel and blot imaging system (Amersham Typhoon 5, Cytiva).
17 (link) Gels were stained with SYPRO Ruby protein gel stain (Invitrogen, Thermo Fisher Scientific) according to the manufacturer's instructions. Images were recorded using the Amersham typhoon gel and blot imaging system (Amersham Typhoon 5, Cytiva).
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