Antibody Panel for Mouse Cell Analysis

The following antibodies were used for flow cytometry: Anti-CD150-PE (TC15–12F12.2; 115904), anti-CD117-PE/Cy7 (2B8; 105814), anti-CD45.2-PE (104; 109807), anti-CD48-Alexa Fluor 647 (HM48–1; 103416) were purchased from BioLegend (San Diego, CA). anti-CD45-APC-eFluor 780 (30-F11; 47–0451-82), anti-Ter119-APC-eFluor 780 (TER-119; 47–5921-82), Streptavidin APC-eFluor 780 (47–4317-82), anti-CD31-PE/Cy7 (MEC13.3; 25–0311-82), anti-CD45.1-FITC (A20; 11–0453-85), anti-B220-APC-eFluor 780 (RA3–6B2; 47–0452-82), anti-CD4-PE/Cy7 (GK1.5; 25–0041-82), anti-CD8a-PE/Cy7 (53–6.7; 25–0081-85), CD51-PE (RMV-7; 13–0512-85), anti-PDGFRα (CD140a)-APC (APA5; 17–1401-81), anti-Ly6A/E(Sca-1)-FITC (D7; 11–5981-82), Anti-CD45-PerCp-Cyanine5.5 (30-F11; 45–0451-82), anti-CD11b (Mac-1)-PE (M1/70; 12–0112-83) were purchased from eBioscience (Thermo Fisher). Anti-lineage panel cocktail (TER-119, RB6–8C5, RA3–6B2, M1/70, 145–2C11 at 1:50 dilution) was from BD Biosciences (559971). Unless otherwise specified, all antibodies were used at a 1:100 dilution. All antibodies were anti-mouse and their specificity was validated in previous studies performed in our laboratory^{3 (link),8 (link),12 (link),27 (link)–29 (link)}.