Cathepsin B Enzymatic Activity Assay

Cathepsin B activity was measured according to the Enzymatic Assay of Cathepsin B protocol provided by Sigma Inc. In brief, cell or tissue lysate was incubated with cathepsin B substrate Nα-CBZ-Arg-Arg-7-amido-4-methylcoumarin at 37 °C, and the fluorescence intensity was determined at 440 nm in a fluorescence microplate reader under excitation at 348 nm (Xiao et al. 2020 (link)).

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Xiao J., Zeng W., Zhang P., Zhou Y, & Fang Q. (2022). Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis. Molecular Medicine, 28, 106.

Publication 2022

Enzymatic Assay of Cathepsin B

Arg arg Cathepsin b Cell Enzymatic assay Fluorescence Tissue

Corresponding Organization : Guilin Medical University

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Variable analysis

independent variables

Incubation of cell or tissue lysate with cathepsin B substrate Nα-CBZ-Arg-Arg-7-amido-4-methylcoumarin

dependent variables

Fluorescence intensity determined at 440 nm in a fluorescence microplate reader under excitation at 348 nm

control variables

Incubation temperature of 37 °C

controls

No positive or negative controls were explicitly mentioned in the provided information.

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