The standard mycotoxin powders, the surfactants Tween-20, and the enzyme stabilizer bovine serum albumin (BSA) were obtained from Sigma-Aldrich (St. Louis, MO, United States). Escherichia coli ER2738 competent cells were purchased from Lucigen Corp. (Middleton, WI, United States). The Universal Probes Supermix was supplied by Bio-Rad (Hercules, CA, United States). DNA polymerase (iTaq), Mg2+, dNTPs, 6× loading buffer, and DNA marker were bought from Takara Bio (Beijing, China). All the other reagents used were of analytical grade or better.
The anti-aflatoxins monoclonal antibody 1C11 (mAb 1C11) and V2–5 phage displaying nanobody specific for 1C11 were produced by our team (Zhang et al., 2009 (link); Wang et al., 2013b (link)). A. flavus strain 3.4408 producing a high level of aflatoxins B1 and B2 was used as a standard strain.
The anti-aflatoxins monoclonal antibody 1C11 (mAb 1C11) and V2–5 phage displaying nanobody specific for 1C11 were produced by our team (Zhang et al., 2009 (link); Wang et al., 2013b (link)). A. flavus strain 3.4408 producing a high level of aflatoxins B1 and B2 was used as a standard strain.
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