Antibody Reagents and Cell Culture

Polybrene and puromycin were provided by Sigma-Aldrich (St. Louis, MO). The anti-GATA4 antibody was purchased from Abcam. The phosphorylated serine antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Other antibodies, as reported previously (20 (link), 31 (link)), were provided by Cell Signaling Technology (Beverly, MA) and SignalAntibody (Shanghai, China). Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), antibiotics, and other cell culture reagents were provided by Gibco-BRL (Suzhou, China).

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Yao J., Wu X.Y., Yu Q., Yang S.F., Yuan J., Zhang Z.Q., Xue J.S., Jiang Q., Chen M.B., Xue G.H, & Cao C. (2022). The requirement of phosphoenolpyruvate carboxykinase 1 for angiogenesis in vitro and in vivo. Science Advances, 8(21), eabn6928.

Publication 2022

Polybrene puromycin anti-GATA4 antibody

Anti antibody Antibiotics Antibodies Antibody Cell culture Eagle Fetal bovine serum Polybrene Puromycin Serine

Corresponding Organization :

Other organizations : Nanjing Medical University, Renji Hospital, Soochow University, Jiangsu University, First People's Hospital of Kunshan

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Protocol cited in 3 other protocols

Variable analysis

independent variables

Polybrene
Puromycin

dependent variables

GATA4 expression
Serine phosphorylation

control variables

Fetal bovine serum (FBS)
Dulbecco's modified Eagle's medium (DMEM)
Antibiotics
Other cell culture reagents

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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