Wound Closure and Cell Migration Assay

Wound closure was performed as previously described^{[17 (link)]}. Mitomycin C (10 μg/mL) was added to the cell culture medium to prevent cell proliferation during migration in the wound healing assay^{[6 (link),17 (link)]}. Wound healing/closure was monitored by assessing the migration of cells for 24 h; photographs were taken at 0 and 24 h with a digital SPOT camera attached to an inverted Nikon phase-contrast microscope. The images were stored in TIF format and processed in Adobe Photoshop (Adobe Systems Inc., Mountain View, CA, USA).

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Senbanjo L.T., AlJohani H., AlQranei M., Majumdar S., Ma T, & Chellaiah M.A. (2020). Identification of sequence-specific interactions of the CD44-intracellular domain with RUNX2 in the transcription of matrix metalloprotease-9 in human prostate cancer cells. Cancer Drug Resistance, 3(3), 586-602.

Publication 2020

Nikon phase-contrast microscope

Assay Camera Cell Cell culture Cell proliferation Cells migration Culture medium Microscope Mitomycin c Phase contrast Wound

Corresponding Organization :

Other organizations : University of Maryland, Baltimore

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Variable analysis

independent variables

Mitomycin C (10 μg/mL) added to the cell culture medium

dependent variables

Migration of cells for 24 h

control variables

Wound closure as previously described

controls

Positive control: Wound closure as previously described
Negative control: Not explicitly mentioned

Annotations

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