Total RNA Extraction from Differentiated Cells

The RNA assay Minikit (Quiagen, Hilden, Germany) with DNAse I treatment was used to extract total RNA, as previously reported [22 (link)]. RNA obtained from differentiated MSCs, SW1353, and CPCs was then quantified spectrophotometrically. The RNA preparation was considered pure when the 260/280 nm absorbance ratio was in the 1.8 to 2.0 range. For gene expression studies related to CPCs, RNA samples were pooled across the six subjects in order to reduce the bias that each individual might introduce.

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Deiana M., Malerba G., Dalle Carbonare L., Cheri S., Patuzzo C., Tsenov G., Moron Dalla Tor L., Mori A., Saviola G., Zipeto D., Schena F., Mottes M, & Valenti M.T. (2019). Physical Activity Prevents Cartilage Degradation: A Metabolomics Study Pinpoints the Involvement of Vitamin B6. Cells, 8(11), 1374.

Publication 2019

Minikit

Assay Cpcs Dnase i Gene expression

Corresponding Organization : University of Verona

Other organizations : Istituti Clinici Scientifici Maugeri

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Variable analysis

independent variables

Differentiated MSCs
SW1353

dependent variables

Gene expression

control variables

RNA obtained from differentiated MSCs, SW1353, and CPCs was quantified spectrophotometrically
The RNA preparation was considered pure when the 260/280 nm absorbance ratio was in the 1.8 to 2.0 range
For gene expression studies related to CPCs, RNA samples were pooled across the six subjects in order to reduce the bias that each individual might introduce

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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